Using IR laser ablation coupled with vacuum capture, a system of fingermark sampling has been developed that could be used for traditional fingerprint analysis and also to capture, filter and analyze biomolecules and trace substances such as DNA or explosives. Laser ablation at three micrometer (µm) was performed in reflection mode with subsequent capture of the ejecta with a filter connected to a vacuum. The laser heated up any moisture present within the surface area, lifting bio-molecules such as DNA off the surface.