Caltech engineers have improved a technique for taking three-dimensional (3-D) microscopic images of tissue, allowing them to see inside living creatures with greater precision than before. The technology, called 3-D photoacoustic microscopy (PAM), bombards tissue with a laser beam. As the energy in the laser light is absorbed, it causes the tissue to vibrate ultrasonically. Those vibrations are picked up by sensors and used to assemble an image of the tissue’s internal structures in a process similar to ultrasound imaging.